My lab is interested in applying proteomics and chemical biology approaches to study protein functions. Current focus is the hnRNPK protein that participates in many cell events, including chromatin recruiting, transcription regulation, RNA splicing and translation regulation. Moreover, amplification of hnRNPK is associated with cancer progression, migration and prognosis. Recently, we demonstrated that two essential arginine methylation of hnRNPK is able to suppress the apoptotic induction of DNA damage in cancer cells. The mechanism regarding how these arginine methylation help cancer cells survive from apoptosis is under study.
Cisd2 is a mitochondrial/ER protein demonstrated by Prof. Ting-Fen Tsai (Department of Life Sciences and Institute of Genome Sciences, NYMU) for its close correlation with aging process in mice model. Our investigation of Cisd2 knockout and transgenic mice using proteomics and metabolomics approaches has identified several pathways that might be associated with the progress of aging. Current focus is to evaluate the potential of metabolites as aging marker.
Andrographolide is a major component of Andrographis paniculata leaves employed for medicinal use, including the anti-inflammatory and neuroprotective activities. Through collaboration with Prof. Shu-Ling Fu (Institute of Traditional Medicine, NYMU), we have demonstrated that andrographolide can transcriptionally down-regulate a triglyceride esterase and thus suppress migration of cancer cells. The mechanism regarding how substrate and product of such esterase affects the biological function of andrographolide is under study.