教師簡介

  • 專任教授
  • 陳俊銘
  • Chun-Ming Chen
  • 02-2826-7000 ext.7339/lab: ext.5721 or 5785
  • cmchen@ym.edu.tw
  • 傳統醫學大樓甲棟1樓R110
  • 專業領域
  • 發育生物學、癌症基因學
  • 實驗室簡介
  • 1. 以轉殖基因小鼠模式探討上皮癌症的形成
    我們以轉殖基因小鼠為模式,利用特定組織專一性的基因剔除或表現系統,廣泛地在單層柱狀上皮細胞或是複層上皮的基底細胞,誘導多個抑癌基 因(如Pten,p53,Apc,Ovca1等)缺失,同時誘導報導基因(如綠螢光蛋白基因)的表現,讓我們能夠在不同器官的柱狀上皮或 基底上皮剔除抑癌基因時,進行研究並追蹤癌症細胞的起始與進展的機制。此外,器官專一性的基因剔除系統也讓我們能更專一地探討特定的癌症 成因。我們目前對於肝癌、大腸直腸癌與攝護腺癌的疾病動物模式有成熟的分析平台,而對於胰臟癌與卵巢癌的起源細胞及其惡 化機制,亦正在積極探討 中。

    2. 以轉殖基因大小鼠模式探討器官的發育與恆定
    我們對於不同層次的幹細胞在胚胎發育過程的器官形成,以及出生後器官的功能維持與恆定有著極大的興趣。藉由轉殖基因大鼠或小鼠為模式,我 們探討幹細胞或前驅細胞的分化對顱顏結構的形成,肝臟的損傷與再生,精子的分化,以及攝護腺上皮與卵巢上皮的恆定等問題。

  • 研究方向
  • The primary interests of my laboratory are to explore the molecular and cellular mechanisms that control the development of embryonic tissues/organs, the homeostasis of adult epithelial tissues, and initiation and progression of cancer. We are particularly interested in the roles of tumor suppressors p53, OVCA1 (Ovarian Cancer Gene 1), PTEN (phosphatase and tensin homolog deleted on chromosome 10) and APC (Adenomatous polyposis coli) in different cancers. To address the roles of these tumor suppressors, we create and develop different mouse genetic resources exhibiting inducible Cre recombinase in different epithelial lineages. Using genetic, biochemical and pharmacological dissection based on our established mouse models, the downstream signaling circuits that act as the key mediators during cancer progression are currently being characterized. Our research outcomes may help us to identify genes or therapeutic targets that block the carcinogenic process.

    In addition, we have focused on the development of the forebrain, the heart, the thymus and the reproductive tracts in the mouse. Using different genetic modified mice, the abnormal phenotypes occurred in the mutant mice are often to be the entry points for us to explore potential mechanisms resulting in developmental defects during mammalian organogenesis.

    Historically, in addition to mice, rats have been used extensively as experimental animals for biomedical research. However, compared to the very large number of genetically modified mice, there are very few mutant rats due to technology limitations. Currently, my laboratory collaborates with Dr. Richard Behringer at the M.D. Anderson Cancer Center who has established a piggyBac-mediated gene-trap mutagenesis system to create a large collection of mutant rats (Furushima et al., 2012, Genetics). The primary goal of this collaborative project is to accelerate and extend the generation of mutant rats, which may enable us to discover novel biological function of the mutated genes and to develop potential disease models in rats.

  • 教授科目
  • 生命科學(二下)分子生物學
    癌症生物學
    小鼠遺傳﹑發育與表現型分析
    論文閱讀與分析方法(三)
    論文閱讀與分析方法(四)
    生命科學教學
  • 獲獎
  • 2005
    吳大猷先生紀念獎
  • 2003
    The Connie and Jim Walter Fellowship in Sarcoma
  • 2001
    M.D. Anderson Cancer Center, Prostate Cancer Research Program Award
  • 經歷
  • 2016-present
    國立陽明大學
    生命科學系暨基因體科學研究所
    教授
  • 2008-2016
    國立陽明大學
    生命科學系暨基因體科學研究所
    副教授
  • 2004-2008
    國立陽明大學
    生命科學系暨基因體科學研究所
    助理教授
  • 2000-2004
    Department of Molecular Genetics, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA
    博士後研究員
  • 1998-2000
    國軍桃園總醫院病理部
    義務役少尉
  • 學歷
  • June 1998
    國立陽明大學
    生化所 博士
  • June 1993
    國立陽明大學
    生化所 碩士
  • June 1991
    中山醫學院
    營養系 學士

五年內代表著作

    * corresponding author

  • Tu, W-L, You, L-R, Tsou, A-P, and Chen, C-M*. Pten haplo-deficiency accelerates liver tumor growth in microRNA122a-null mice via expansion of periportal hepatocyte-like cells. Am J Pathol 2018, In press.
  • Chan, C-H, Chen, C-M., Wu Lee, Y-H, and You, L-R.* DNA Damage, Liver Injury, and Tumorigenesis: Consequences of DDX3X Loss. Mol Cancer Res, 2018, accepted.
  • Hsu W-H, Chen C-M, You L-R*. COUP-TFII is required for morphogenesis of the neural crest-derived tympanic ring. Sci Rep. 2017, 7: 12386.
  • Wang C-Y, Tang M-C, Chang W-C, Furushima K, Jang C-W, Behringer RR, Chen C-M*. PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility. Biol Reprod. 2016; 95: 51, 1-9.
  • Li H-K, Mai R-T, Huang H-D, Chou C-H, Chang Y-A, Chang Y-W, You L-R, Chen C-M, Wu Lee Y-H*. DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma. Sci Rep. 2016;6:28637.
  • Chen C-Y, Chan C-H, Chen C-M, Tsai Y-S, Tsai T-Y, Wu Lee Y-H, You L-R*. Targeted Inactivation of Murine Ddx3x: Essential Roles of Ddx3x in Placentation and Embryogenesis. Hum Mol Genet. 2016; 25: 2905-2922.
  • Lu T-L and Chen C-M*. ß-Catenin in epithelial tumorigenesis. Aging 2015; 7: 467-468.
  • Liang C-C, Lu T-L, Yu Y-R, You L-R and Chen C-M*. ß-Catenin activation drives thymoma initiation and progression in mice. Oncotarget 2015; 6:13978-13993.
  • Lu T-L and Chen C-M* .Differential Requirements for ß-Catenin in Murine Prostate Cancer Originating from Basal versus Luminal Cells. J Pathol. 2015; 236: 290-301.
  • Yu Y-R, You L-R, Yan Y-T and Chen C-M*. Role of OVCA1/DPH1 in craniofacial abnormalities of Miller-Dieker Syndrome. Hum Mol Genet, 2014; 23:5579-5596.
  • Liang C-C, You L-R, Yen J-J, Liao N-S, Yang-Yen H-F, Chen C-M*. Thymic epithelial beta-catenin is required for adult thymic homeostasis and function. Immunol Cell Biol 2013; 91: 511-23.
  • Lu T-L, Huang Y-F, You L-R, Chao N-C, Su F-Y, Chang J-L and Chen C-M*. Conditionally Ablated Pten in Prostate Basal Cells Promotes Basal-to-Luminal Differentiation and Causes Invasive Prostate Cancer in Mice. Am J Pathol 2013; 182: 975-991.
  • Cheng T-L, Wu Y-T, Lai C-H, Kao Y-C, Kuo C-H, Liu S-L , Hsu Y-Y, Chen P-K, Cho C-F, Wang K-C, Lin W-L, Chang B-I, Chen C-M, Weiler H, Shi GY, Wu HL* Thrombomodulin Regulates Keratinocyte Differentiation and Promotes Wound Healing. J Invest Dermatol 2013; 133:1638-1645.
  • Hsu W-H, Yu Y-R, Hsu S-H, Yu W-C, Chu Y-H, Chen,Y-J, Chen C-M and You L-R* The Wilms’ tumor suppressor Wt1 regulates Coronin 1B expression in the epicardium. Exp Cell Res 2013; 319: 1365-1381.
  • Chiang M-F, Yang S-Y, Lin I-Y, Hong J-B, Lin S-J, Ying H-Y, Chen C-M, Wu S-Y, Liu F-T, Lin K-I*. Inducible deletion of the Blimp-1 gene in adult epidermis causes granulocyte-dominated chronic skin inflammation in mice. Proc Natl Acad Sci USA 2013; 110: 6476-6481.