教師簡介

  • 專任教授
  • 羅清維
  • Ching-Wei Luo
  • 02-2826-7000 ext. 7185/lab: ext. 5769 or 5779
  • 傳統醫學大樓甲棟1樓R111
  • 實驗室網頁
  • http://www.ym.edu.tw/~cwluo/
  • 專業領域
  • 分子內分泌學、訊息傳導、生殖系統癌症機轉
  • 研究方向
  • The functions of various hormones are mediated through their corresponding receptors, which serve as communicators between extracellular and intracellular signals. Among the present pharmaceutical products, more than 90% of marketed drugs are hormone derivatives or molecules designed to target the mechanisms conducted by hormone-receptor interactions. Therefore, in our lab we aim to discover the novel hormones and characterize the molecular actions of hormone receptors. Several strategies and research goals were briefly described below.

    Project 1. Molecular mechanisms of cancers related to reproductive system.

    We currently have two running cancer projects. (1) Ovarian cancer: Ovarian cancer is the third most common but the first leading cause of death from gynecologic malignancies among women in Taiwan. In this project, we will try to characterize the differential expression of genes by in vivo cancer selection. We aim to develop nano-drugs for translational medicines through collaboration with our team groups. (2) Uterine cancer: Endometrial carcinoma is the most common malignancy of the female genital tract in western countries and shows an increasing incidence rate in Taiwan. In this project, we now focus on the effects of GPCR signaling on tumorigenesis.

    Project 2. TGF-beta/BMP signaling in postnatal gonadal development.

    Many members of the TGF-beta/BMP family play crucial roles in controlling postnatal gonadal development such as follicle selection and spermatogenesis. However, their distribution, molecular mechanism as well as counterplaying antagonists have not been clearly elucidated. By establishing their temporal and spatial profiles and characterizing their signaling in the gonads, we aim to develop approaches in treatment of infertility.

    Project 3. Hormonal actions on ovarian development.

    Mammalian ovary is the organ that contains the largest numbers of endocrine as well as paracrine factors. The communication between these signaling molecules and receptors expressed in the oocyte, granulosa cells and theca/interstitial cells governs the follicular growth and ovarian development. Therefore, we are interested in exploring the potential function and physiological regulation of the novel hormones found in the ovary.

    Project 4. Functional genomic approaches for novel hormone discovery.

    Using computational tools to analyze the massive protein databases in species, homologies among polypeptide hormones can be sorted and placed into families. We have identified several novel endocrine genes based on evolutionary conservation. Following the gene identification, the recombinant hormones will be generated and the potential functions will be studied.

  • 教授科目
  • 生命科學(二下):分子生物學
    論文閱讀與分析方法(一)
    論文閱讀與分析方法(二)
    生命科學技術
    生命科學教學
  • 經歷
  • Feb, 2017-present
    國立陽明大學
    生命科學系暨基因體科學研究所
    教授
  • Aug, 2010-Jan, 2017
    國立陽明大學
    生命科學系暨基因體科學研究所
    副教授
  • Aug, 2006-Jul, 2010
    國立陽明大學
    生命科學系暨基因體科學研究所
    助理教授
  • Nov, 2005-Jul, 2006
    國立陽明大學生命科學系
    助理教授
  • May 2002-Oct, 2005
    Stanford Univ. Medical Center
    博士後研究員
  • Jul, 2001-Apr, 2002
    中央研究院生物化學所
    博士後研究員
  • Jan, 2001-Jun, 2001
    國立臺灣大學生化科學所
    博士後研究員
  • 學歷
  • Sep, 1996-Dec, 2000
    國立臺灣大學
    生化科學研究所 博士
  • Sep, 1992-Jun, 1994
    國立臺灣大學
    生化科學研究所 碩士
  • Sep, 1988-Jun, 1992
    國立臺灣大學
    農業化學系 學士

代表著作

  • Wu FJ, Lin TY, Sung LY, Chang WF, Wu PC, Luo CW*. (2017) BMP8A sustains spermatogenesis by activating both SMAD1/5/8 and SMAD2/3 in spermatogonia. Science Signaling. 10 (477). pii: eaal1910. doi: 10.1126/scisignal.aal1910. Featured as (Cover Story). (Full text). (Free Reprint).
  • Huang WL, Li Z, Lin TY, Wang SW, Wu FJ, Luo CW*. (2016) Thyrostimulin-TSHR signaling promotes the proliferation of NIH:OVCAR-3 ovarian cancer cells via trans-regulation of the EGFR pathway. Scientific Reports. 6:27471. doi: 10.1038/srep27471 (PubMed).
  • Lin TY, Wu FJ, Chang CL, Li Z, Luo CW*. (2016) NMU signaling promotes endometrial cancer cell progression by modulating adhesion signaling. Oncotarget. 3. doi: 10.18632/oncotarget.7169. (PubMed).
  • Lin TY, Huang WL, Lee WY, Luo CW*. (2015) Identifying a Neuromedin U Receptor 2 Splice Variant and Determining Its Roles in the Regulation of Signaling and Tumorigenesis In Vitro. PLoS One.10(8):e0136836. (PubMed).
  • Hsu PJ, Wu FJ, Kudo M, Hsiao CL, Hsueh AJ, Luo CW*. (2014) A naturally occurring lgr4 splice variant encodes a soluble antagonist useful for demonstrating the gonadal roles of lgr4 in mammals. PLoS One, 9(9),e106804. doi: 10.1371/journal.pone.0106804. (PubMed).
  • Deng C, Reddy P, Cheng Y, Luo CW, Hsiao CL, Hsueh AJ*. (2013) Multi-functional norrin is a ligand for the LGR4 receptor. Journal of Cell Science, 126, 2060-2068. (PubMed).
  • Lin TY, Wu FJ, Lee WY, Hsiao CL, Luo CW* (2013) Ovarian regulation of neuromedin U and its local actions in the ovary, mediated through neuromedin U receptor 2. Am J Physiol Endocrinol Metab., 304, E800-9. (PubMed).
  • Lee WY, Wang CJ, Lin TY, Hsiao CL, Luo CW* (2013) CXCL17, an orphan chemokine, acts as a novel angiogenic and anti-inflammatory factor. Am J Physiol Endocrinol Metab., 304, E32-40. (PubMed).
  • Hung WT, Wu FJ, Wang CJ, Luo CW* (2012) DAN (NBL1) Specifically Antagonizes BMP2 and BMP4 and Modulates the Actions of GDF9, BMP2, and BMP4 in the Rat Ovary. Biology of Reproduction. 86, 1-9. (PubMed).
  • Tseng HC, Tang JB, Sudhakar Gandhi PS, Luo CW, Ou CM, Tseng CJ, Lin HJ*, Chen YH*. (2012) Exclusive expression of a membrane-bound Spink3-interacting serine protease-like protein TESPL in mouse testis. Amino Acids. 42, 951-960. (PubMed).
  • Ou CM, Lin SR, Lin HJ, Luo CW*, Chen YH.* (2010) Exclusive expression of a membrane-bound Spink3-interacting serine protease-like protein TESPL in mouse testis. J Cell Biochem. 110, 620-629. (PubMed).
  • Sun SC, Hsu PJ, Wu FJ, Li SH, Lu CH, Luo CW* (2010) Thyrostimulin, but not thyroid-stimulating hormone, acts as a paracrine regulator to activate thyroid-stimulating hormone receptor in the mammalian ovary. J Biol Chem. 285, 3758–3765 (PubMed)- selected for Faculty of 1000 Medicine.
  • Wang CJ, Hsu SH, Hung WT, Luo CW* (2009) Establishment of a chimeric reporting system for the universal detection and high-throughput screening of G protein-coupled receptors. Biosensors and Bioelectronics. 24, 2298-2304. (PubMed).
  • Cho CC, Luo CW, Hsu CH. (2008) Crystallization and preliminary X-ray diffraction analysis of PAT, an acetyltransferase from Sulfolobus solfataricus. Acta Crystallogr Sect F Struct Biol Cryst Commun. F64, 1049–1051. (PubMed).
  • Zhang JV, Jahr H, Luo CW, Klein C, Van Kolen K, Ver Donck L, De A, Baart E, Li J, Moechars D, Hsueh AJ. (2008) Obestatin induction of early response gene expression in gastrointestinal and adipose tissues and the mediatory role of GPR39. Mol Endocrinol. 22, 1464-1475. (PubMed).
  • Dai L, Dewey EM, Zitnan D, Luo CW, Honegger HW, Adams ME. (2008) Identification, developmental expression, and functions of bursicon in the tobacco hawkmoth, Manduca sexta. Journal of comparative neurology. 506, 759-774. (PubMed).
  • Hsu SH, Luo CW* (2007) Molecular Dissection of G protein Preference Using G{alpha}s Chimeras Reveals Novel Ligand Signaling of GPCRs. Am J Physiol Endocrinol Metab. 293, E1021-9. (PubMed).
  • Lin HJ, Luo CW, Wang CY, Chen YH. (2006) Epitope topology and removal of mouse acrosomal plasma membrane by P12-targeted immunoaggregation. Biochemical and biophysical research communications. 349, 284-288. (PubMed).
  • Luo CW, Hsueh AJ. (2006) Genomic analyses of the evolution of LGR genes. Chang Gung Medical Journal. 29, 2-8. (PubMed).
  • Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ. (2005) Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science. 310, 996-999. (PubMed).
  • Mazerbourg S, Sangkuhl K, Luo CW, Sudo S, Klein C, Hsueh AJ. (2005) Identification of receptors and signaling pathways for orphan bone morphogenetic protein/growth differentiation factor ligands based on genomic analyses. J Biol Chem. 280, 32122-32132. (PubMed).
  • Luo CW, Dewey EM, Sudo S, Ewer J, Hsu SY, Honegger HW, Hsueh AJ. (2005) Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2. Proc Natl Acad Sci U S A. 102, 2820-2825. (PubMed).
  • Luo CW, Pisarska MD, Hsueh AJ. (2005) Identification of a stanniocalcin paralog, STC2, in fish and the paracrine actions of STC2 in the mammalian ovary. Endocrinology. 146, 469-476. (PubMed).
  • Lin HJ, Lee CM, Luo CW, Chen YH. (2005) Functional Preservation of duplicated pair for RSVS III gene in the REST locus of rat 3q42. Biochem Biophys Res. Commun. 326, 355-363. NSC92-2311-B-002-019 and NSC92-2311-B-001-089. (PubMed).
  • Morita H, Mazerbourg S, Bouley DM, Luo CW, Kawamura K, Yoshimitsu K, Baribault H, Tian H, Hsueh AJ. (2004) Neonatal lethality of LGR5 null mice is associated with ankyloglossia and gastrointestinal distension. Mol Cell Biol. 22, 9736-9743. (PubMed).
  • Luo CW, Kawamura K, Klein C, Hsueh AJ. (2004) Paracrine regulation of ovarian granulosa cell differentiation by stanniocalcin 1 (STC1): mediation through specific STC1 receptors. Mol Endocrinol. 18, 2085-2096. (PubMed).
  • Luo CW, Lin HJ, Gopinath SC, Chen YH. (2004) Distinction of sperm-binding site and reactive site for trypsin inhibition on p12 secreted from the accessory sex glands of male mice. Biol Reprod. 70, 965-971. NSC91-2311-B001-076/NSC91-2311-B002-049. (PubMed).
  • Lin HJ, Luo CW, Chen YH. (2002) Localization of the transglutaminase cross-linking site in SVS III, a novel glycoprotein secreted from mouse seminal vesicle. J Biol Chem. 277, 3632-3639. NSC90–2311-B-002-023/NSC90–2311-B-001-049. (PubMed).
  • Luo CW, Lin HJ, Chen YH. (2001) A novel heat-labile phospholipid-binding protein, SVS VII, in mouse seminal vesicle as a sperm motility enhancer. J Biol Chem. 276, 6913-6921. (SCI), NSC89-2311-B-002-038/NSC89-2311-B-001-106. (PubMed).