教師簡介

  • 專任副教授
  • 范秀芳
  • Hsiu-Fang Fan
  • 02-2826-7000 ext. 7941/lab: ext. 6210
  • hffan2@ym.edu.tw
  • 圖書資訊暨研究大樓7樓R702
  • 專業領域
  • Biophysical chemistry, Single-molecule imaging, Optical and fluorescence microscopy, Magnetic tweezers, Protein-DNA interactions (DNA recombinases, nucleosome remodeler, histone chaperones)
  • 研究方向
    • Mechanism of DNA repair mechanism, site-specific recombination, nucleosome remodeling.
    • Developing optical system to study biological system in vitro and invivo.
  • 教授科目
  • 化學原理(一)
    化學原理(二)
    化學原理實驗
    分析生化學
    生物影像分析在細胞生物學的應用
    生化及細胞分子生物學(一)
    光譜分析於生物上的應用
    專題討論(碩)
  • 經歷
  • Feb, 2015-present
    國立陽明大學
    生命科學系暨基因體科學研究所
    副教授
  • Feb, 2010-Jan, 2015
    國立陽明大學
    生命科學系暨基因體科學研究所
    助理教授
  • Jan, 2009-Jan, 2010
    國立臺灣大學
    化學博士後研究員
    with Professor K. C. Lin
  • Dec, 2007-Nov, 2008
    比利時荷蘭語天主教魯汶大學
    博士後研究員
    with Professor Yves Engelborghs
  • Nov, 2006-Oct, 2007
    加拿大麥基爾大學
    化學博士後研究員
    with Professor H. W. Li
  • Jul, 2006-Sep, 2006
    國立臺灣大學
    化學博士後研究員 with Professor K. C. Lin
  • 學歷
  • 2000-2006
    國立臺灣師範大學
    化學系 博士
    with Professor K. C. Lin
  • 1996-2000
    國立臺灣師範大學
    化學系 學士

代表著作

    2010-present

  • Single molecule analysis of PhiC31 integrase-mediated site-specific recombination by tethered particle motion. Nucleic Acids Res. 2016, 44(12):5849-5860. Hsiu-Fang Fan*; Tao-Shih Hsieh; Chien-Hui Ma; Makkuni Jayaram
  • Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination. Nucleic Acids Res. 2015, March 12 on line. Hsiu-Fang Fan*; Yong-Song Cheng; Chien-Hui Ma; Makkuni Jayaram
  • Histone chaperone-mediated nucleosome assembly process. PLoS One 2015, 6(7), e21359. Hsiu-Fang Fan*; Zi-Ning Liu; Sih-Yao Chow; Yi-Han Lu; Hsin Li
  • Organization of DNA partners and strand exchange mechanisms during Flp site-specific recombination analyzed by difference topology, single molecule FRET and single molecule TPM. J. Mole. Biol. 2014, 426, 793-815, Chien-Hui Ma; Yen-Tin Liu; Christos G; Savva, Paul A. Rowley; Brian Cannon; Hsiu-Fang Fan; Rick Russell; Andreas Holzenburg; Makkuni Jayaram*.
  • Using single-molecule approaches to study the archaeal DNA binding protein Alba1. Biochemistry 2013, 52, 7714-7722, Yen-Wen Lu; Tao Huang; Cheng-ting Tsai; Yu-Yung Chang; Hung-Wen Li; Chun-Hua Hsu; Hsiu-Fang Fan*
  • Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int. Nucleic Acids Res. 2013, 41, 7031-47. Hsiu-Fang Fan*; Chien-Hui Ma; Makkuni Jayaram*.
  • The characterization of the nuclear dynamics of syntenin-2, a PIP(2) binding PDZ protein. Cytometry A. 2013, 83, 866-875. Annelies Geeraerts; Hsiu-Fang Fan; Pascale Zimmermann; * Yves Engelborghs*
  • Metabolism of HeLa cells revealed through autofluorescence lifetime upon infection with enterohemorrhagic Escherichia coli. J Biomed Opt. 2012, 10, 101503. Tatyana Yu. Buryakina ; Pin-Tzu Su ; Wan-Jr Syu ; C. Allen Chang ; Hsiu-Fang Fan ; Fu-Jen Kao*
  • Real-time single-molecule tethered particle motion experiments reveal the kinetics and mechanisms of Cre-mediated site-specific recombination. Nucleic Acids Res. 2012 40, 6208-22. Hsiu-Fang Fan*
  • Developing single-molecule TPM experiments for direct observation of successful; RecA-mediated strand exchange reaction. PLoS One 2011, 6(7), e21359. Hsiu-Fang Fan; Micheal M. Cox; Hung-Wen Li*
  • Photoinduced electron transfer of oxazine 1/TiO2 nanoparticles at single molecule level by using confocal fluorescence microscopy. Langmuir 2010, 26,9050-9060, Yi-Ju Chen; Hsin-Yu Tzeng; Hsiu-Fang Fan; Ming-Shiang Chen; Jer-Shing Huang; King-Chuen Lin*
  • Kinetics and Thermodynamic Investigation of Rhodamine B Adsorption at Solid/Solvent Interfaces Using Evanescent-Wave Cavity Ring-Down Spectroscopy. Anal. Chem. 2010, 82, 868-877. Ming-Shiang Chen; Hsiu-Fang Fan; King-Chuen Lin.*

    2004-2010

  • Studying RecBCD helicase translocation along Chi-DNA using tethered particle motion with a stretching force. Biophys. J. 2009, 96, 1875-1883. Hsiu-Fang Fan; Hung-Wen Li*
  • Characterization of two types of silanol groups on fused-silica surfaces using evanescent-wave cavity ring-down spectroscopy. Anal. Chem. 2007, 79, 3654-3661. Hsiu-Fang Fan; Fuping Li; Richard N. Zare; King-Chuen Lin*
  • Molecular Adsorption at Silica/CH3CN Interface Probed by Using Evanescent Wave Cavity Ring-down Absorption Spectroscopy: Determination of Thermodynamic Properties. Anal. Chem. 2006, 78, 3583-3590. Hsiu-Fang Fan; Chien-Yi Hung; King-Chuen Lin*
  • Catalytic Isomerization of Quadricyclane in Heterogeneous Reaction Using Fourier Transform Near Infrared Absorption Spectroscopy: Diffusion, Conversion and Temperature Effect. J. Phys. Chem. B. 2006, 110, 5563-5569. Hsiu-Fang Fan; Chia-Yu Chang; Thou-Long Chin; Tong-Ing Ho; King-Chuen Lin*
  • Kinetics of Catalytic Isomerization of Quadricyclane to Norbornadiene Using Near Infrared Absorption Spectroscopy: Conversion Rate and Diffusion Motion in Heterogeneous Reaction. J. Phys. Chem. B. 2004, 108, 9364-9370. Hsiu-Fang Fan; Thou-Long Chin; King-Chuen Lin*