教師簡介

  • 專任副教授
  • 張欣暘
  • Hsin-Yang Chang
  • 02-2826-7000 ext.7168/lab: ext. 5789 or 5771 or 5726
  • hychang7@ym.edu.tw
  • 傳統醫學大樓甲棟1樓R108
  • 專業領域
  • 結構生物學 (Structural biology)、X-Ray蛋白晶體學(X-ray crystallography)、酵素動力學 (Enzyme kinetics)
  • 研究方向
  • 我們主要研究目標是希望能夠以分子層次的角度上去探討細胞膜蛋白如何進入生物膜的生理機制。大多數真核細胞膜蛋白質首先被插入內質網, 此過程稱為共同轉譯途徑(co-translational pathway)。然而,有一類在生理功能上比較特別的膜蛋白,屬於尾端固定(tail-anchored, TA)膜蛋白家族,它們並不藉由共同轉譯途徑完成此步驟,而是經由另一個稱為後轉譯途徑(post-translational pathway) 的特殊新途徑完成。此途徑只有在真核細胞如哺乳動物、植物和酵母等才有。之前的研究也已經發現內質網、粒線體和葉綠體的TA膜蛋白在生物生長和發育過程中,對於代謝調控、 疾病以及環境逆激等反應扮演重要的角色,然而其生理分子機制卻仍然不清楚。我們最近解出綠藻ArsA1蛋白質3D結構,了解到特定TA- 膜蛋白運如何運送至葉綠體和粒線體外膜。我們實驗室主要是利用X-ray蛋白晶體繞射學、酵素動力學,以及生物化學並結合功能性和突變分 析等方法來探討此特殊後轉譯途徑的生理機制。這項工作預期會在生物物理、化學以及細胞訊息通路的調節上有相當廣泛的應用。對於動物TA膜 蛋白所引發的致病分子機制以及植物光合作用效率提升將有更進一步了解。





  • 教授科目
  • 生物化學
    生命科學總論
    結構生物學
  • 經歷
  • Aug. 2020-present
    國立陽明大學
    生命科學系暨基因體科學研究所
    副教授
  • Aug. 2019-Jul. 2020
    國立中山大學
    海洋生物科技暨資源學系
    副教授
  • Aug. 2015- Jul. 2019
    國立中山大學
    海洋生物科技暨資源學系
    助理教授
  • Sep. 2013- Jul. 2015
    中央研究院生化所
    博士後
  • Jun. 2011- Aug. 2013
    美國芝加哥大學生物化學暨分子生物所
    博士後
  • Jun. 2010- Jun. 2011
    美國伊利諾大學香檳分校生物化學所
    博士後
  • Jan. 2000- Jul. 2004
    中央研究院生化所
    國防科技役助理
  • 學歷
  • 2010 美國伊利諾大學香檳分校
    (University of Illinois at Urbana-Champaign)
    生物化學所 博士


代表著作

    期刊發表

  • HC Lin, WH Li, CC Chen, TH Cheng, YH Lan, MD Huang, WM Chen, JS Chang, HY Chang*(2020) Diverse enzymes with industrial applications in four thraustochytrid genera. Front. Microbiol., https://doi.org/10.3389/fmicb.2020.573907.
  • CY Chiang, CC Chou, HY Chang, MF Hsu, PJ Pao, MH Chiang, AHJ Wang (2020) Biochemical and molecular dynamics studies of archaeal polyisoprenyl pyrophosphate phosphatase from Saccharolobus solfataricus. Enzyme Microb. Technol. doi.org/10.1016/ j.enzmictec.2020.109585
  • HL Yeh, TH Lin, CC Chen, TX Cheng, HY Chang, TM Lee (2019) Monodehydroascorbate reductase plays a role in the tolerance of Chlamydomonas reinhardtii to photooxidative stress. Plant Cell Physiol. 60: 2167–2179.
  • TW Lin, CC Chen, SM Wu, YC Chang, YC Li, YW Su, CD Hsiao, HY Chang*. (2019) Structural analysis of chloroplast tail-anchored membrane protein recognition by ArsA1, The Plant Journal, 99:128-143.
  • HY Chang*, TP Ko, YC Chang, KF Huang, CY Lin, HY Chou, CY Chiang, HJ Tsai. (2019) Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni, Int. J. Biol. Macromol. 130: 675-684.
  • HY Chang*, ST Lin, TP Ko, SM Wu, TH Lin, YC Chang, KF Huang, TM Lee. (2017) Enzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress, Plant Physiol. Biochem. 120: 144-155.
  • M Maestre-Reyna, SM Wu, YC Chang, CC Chen, A Maestre-Reyna, AHJ Wang, HY Chang*. (2017) In search of tail-anchored protein machinery in plants: reevaluating the role of arsenite transporters, Scientific Report 7:46022 | DOI: 10.1038/srep46022.
  • HY Chang, CC Chou, ML Wu, AHJ Wang. (2017) Expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus, Protein Expr. Purif. 133: 121-131.
  • C von Ballmoos, I Smirnova, F Poiana, N Gonska, HY Chang, RB Gennis, P Brzezinski, P Ädelroth. (2017) Dynamics of the KB proton pathway in cytochrome ba3 from Thermus thermophiles, Isr. J. Chem. 57: 1-14. DOI: 10.1002/ijch.201600136.
  • ST Lin, CW Chiou, YL Chu, YH, YF Tseng, YC Chen, HJ Chen, HY Chang, TM Lee. (2016) Enhanced ascorbate regeneration via dehydroascorbate reductase confers tolerance to photo-oxidative stress in Chlamydomonas reinhardtii. Plant Cell Physiol. 10: 2104-2121.
  • A Mateja, M Paduch, HY Chang, A Szydlowska, AA Kossiakoff, RS Hegde, RJ Keenan. (2015) Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science. 347: 1152-1155.
  • HY Chang, CC Chou, MF Hsu, AHJ Wang. (2014) Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli. J. Biol. Chem. 289: 18719-18735.
  • I Smirnova, HY Chang, C von Ballmoos, P Ädelroth, RB Gennis, P Brzezinski. (2013) Single mutations that redirect internal proton transfer in the ba3 oxidase from Thermus thermophilus. Biochemistry 52: 7022-7030.
  • HY Chang, SK Choi, AS Vakkasoglu, Y Chen, J Hemp, JA Fee, RB Gennis. (2012) Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus. Proc Natl Acad Sci U S A 109: 5259-5264.
  • HY Chang, Y Ahn, LA Pace, MT Lin, YH Lin, RB Gennis. (2010) The diheme cytochrome c4 from Vibrio cholerae is a natural electron donor to the cbb3-type cytochrome c oxidase. Biochemistry 49: 7494-7503.
  • I Smirnova, J Reimann, C von Ballmoos, HY Chang, RB Gennis, JA Fee, P Brzezinski, P Ädelroth. (2010) Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba3 cytochrome c oxidase from Thermus thermophilus. Biochemistry 49: 7033-7049.
  • HY Chang, J Hemp, Y Chen, JA Fee, RB Gennis. (2009) The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proc Natl Acad Sci U S A 106: 16169-16173.
  • TH Kuo, SC Chuang, SY Chang, PH Liang. (2006) Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping. Biochem. J. 393: 757-766.
  • APC Chen, SY Chang, YC Lin, YS Sun, CT Chen, AHJ Wang, PH Liang. (2005) Substrate and product specificities for cis-type undecaprenyl pyrophosphate synthase. Biochem. J. 386: 169-176.
  • SY Chang, TP Ko, APC Chen, AHJ Wang, PH Liang. (2004) Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies. Protein Sci. 13: 971-978.
  • SY Chang, TP Ko, PH Liang, AHJ Wang. (2003) Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and Triton. J. Biol. Chem. 278: 29298-29307.
  • SY Chang, YK Chen, AHJ Wang, PH Liang. (2003) Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase. Biochemistry. 42: 14452-14459.
  • CE Atreya, EF Johnson, J Williamson, SY Chang, PH Liang, KS Anderson. (2003). Probing electrostatic channeling in protozoal bifunctional thymidylate synthase-dihydrofolate reductase using site-directed mutagenesis. J. Biol. Chem. 278: 28901- 28911.
  • SY Chang, PC Tsai, CS Tseng, PH Liang. (2001) Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli. Prot. Exp. Puri. 23: 432-43.