教師簡介

  • 專任教授
  • 高閬仙
  • Lung-Sen Kao
  • 02-2826-7000 ext.6252
  • 圖書資訊暨研究大樓7樓R708
  • 專業領域
  • 分子細胞神經生物學、細胞內鈣離子調控、神經傳導物質分泌機制、神經退化性疾病病理
  • 研究方向
  • Our research interests have focused on the molecular mechanism of the regulated secretory process and the pathogenesis of Parkinson’s disease (PD). Our laboratory has been using chromaffin cells and PC12 cells as model systems to study the molecular machinery of Ca2+-dependent exocytosis for many years. The accumulated information and experience in the study of these catecholaminergic cells have prompted our study moving from the mechanistic study in model systems to the understanding of the dopamine system in brain under physiological and pathological conditions, mainly PD in which the degeneration of dopaminergic neurons is the primary cause. Our research focuses on the role of α-synuclein in the pathogenesis of PD. The specific projects currently working in the laboratory are:

    • Role of α-synuclein in regulated secretion - We have performed a detailed characterization of the role of Rab3A and its relationship with Munc13 and Munc18 during vesicle priming before the occurrence of exocytosis based on the total internal reflection fluorescence microscope images obtained. α-Synuclein is a protein that present in Lewy body and Lewy neurites. Evidence supports that α-synuclein is involved in neurotransmitter release by regulated secretion. The goal is to clearly define how α-synuclein interacts with the individual molecules and where α-synuclein acts in the various steps occurred during exocytosis.
    • Association between the physiological function of α-synuclein and the degeneration of DA neurons - We have characterized the Ca2+ signaling pathways in primary cultured mouse mesencephalic dopaminergic neurons and established two animal models for the study of pathogenesis of PD: an “aging” version of the well accepted MPP+-induced dopaminergic neuron degeneration mouse model and a Zn2+ and dopamine-induced striatal dopamine depletion mouse model based on our previous results. We are using the two mouse models to study the association between the physiological function of α-synuclein and the pathogenesis of PD.
  • 教授科目
  • 生命科學(二上):生物化學
    生命科學(二下):細胞生物學
    學術研究倫理
  • 經歷
  • Feb, 2000–present
    國立陽明大學
    生命科學系暨基因體科學研究所
    教授
  • Jun, 2013-Dec , 2017
    國立陽明大學
    副校長
  • Dec 1, 2017-Dec 29, 2017
    國立陽明大學
    代理校長
  • Jun, 2013-Jul, 2015
    國立陽明大學
    教務長
  • Sep, 2009–Feb, 2015
    國立陽明大學腦科學研究中心
    主任
  • Apr, 2008–May, 2013
    國立陽明大學生命科學院
    院長
  • Feb, 2000–2006
    國立陽明大學
    生命科學系暨基因體科學研究所
    所長兼系主任
  • 1992–Feb, 2000
    中央研究院生物醫學科學研究所
    研究員
  • 學歷
  • 1983
    美國麻州州立大學
    生化 博士
  • 1977
    國立臺灣大學
    農業化學 碩士
  • 1975
    國立臺灣大學
    農業化學 學士

代表著作

  • Yang TC, Wu PC, Chung IF, Jiang JH, Fann MJ, and Kao LS*. Cell Death Caused by the Synergistic Effects of Zn2+ and Dopamine is Mediated by a Stress Sensor Gene Gadd45b - Implication in the Pathogenesis of Parkinson’s Disease. J. Neurochem. 2016; Accept. Array data available.
  • Wu PC, Kao LS*. Calcium regulation in mouse mesencephalic neurons - Differential roles of Na+/Ca2+ exchanger, mitochondria and endoplasmic reticulum. Cell Calcium. 2016; doi:10.1016/j.ceca.2016.03.008.
  • Fuh JL, Chung MY, Yao SC, Chen PK, Liao YC, Hsu CL, Wang PJ, Wang YF, Chen SP, Fann CS, Kao LS*, Wang SJ*. (2015) Susceptible genes of restless legs syndrome in migraine. Cephalalgia. pii: 0333102415620907.
  • Soong BW, Huang YH, Tsai PC, Huang CC, Pan HC, Lu YC, Chien HJ, Liu TT, Chang MH, Lin KP, Tu PH, Kao LS*, Lee YC*. Exome sequencing identifies GNB4 mutations as a cause of dominant intermediate Charcot-Marie-Tooth disease. Am. J. Human Genet. 2013; 92: 422-430.
  • Huang CC, Yang DM, Lin CC*, Kao LS*. Involvement of Rab3A in vesicle priming during exocytosis: interaction with Munc13-1 and Munc18-1. Traffic 2011; 12:1356–1370.
  • Wu PC, Fann MJ, Kao LS*. Characterization of Ca2+ Signaling Pathways in Mouse Adrenal Medullary Chromaffin Cells. J. Neurochem. 2010; 112(5):1210-1222.
  • Yang YC, Fann MJ, Chang WH, Tai LH, Jiang JH, Kao LS*. Regulation of sodium-calcium exchanger activity by creatine kinase under energy-compromised conditions. J. Biol. Chem. 2010; 285(36):28275-28285.
  • Pan CY, Tsai LL, Jiang JH, Chen LW, Kao LS*. (2008) The co-presence of Na+/Ca2+-K+ exchanger and Na+/Ca2+ exchanger in bovine adrenal chromaffin cells. J. Neurochem. 107(3):658-67.
  • Lin CC, Huang CC, Lin KH, Cheng KH, Yang DM, Tsai YS, Ong RY, Huang YN, Kao LS*. (2007) Visualization of Rab3A dissociation during exocytosis: a study by total internal reflection microscopy. J. Cell Physiol. 211(2):316-26.
  • Lo HS, Chiang HC, Lin MY, Chiang HY, Chu YC and Kao LS* (2004) Synergistic effects of dopamine and Zn2+ on the induction of PC12 cell death and dopamine depletion in the striatum: possible implication in the pathogenesis of Parkinson's disease. Neurobiol. Dis. 17, 54-61.