教師簡介

  • 合聘教授
  • 蔡世峰
  • Shih-Feng Tsai
  • 02-2826-7000 ext.6066
  • petsai@nhri.org.tw

  • 專業領域
  • 基因體醫學、人類遺傳學
  • 研究方向
  • 過去數年來,我們對正常肝臟與肝癌組織之基因表現深感興趣,本實驗室應用先進科技,大量分析基因之表現。研究結果顯示蛋白激脢在不同生 理病理狀況下有不同的表現型式。我們也透過團隊合作,建立研究肝臟相關研究所需之基因庫材料並且分析了數千筆肝臟之基因序列。這些資訊可 供研究者參考,從中找尋研究的新方向。最近,我們更致力建立百萬鹼基級之基因定序能力,除了協助搜尋可能之抑癌基因外,亦可對人類基因組 計劃有所貢獻。

    人類基因體計畫 (The Human Genome Project),自1998年進入一個高峰。藉由先前遺傳圖譜 (genetic map)及物理圖譜 (physical map) 之基礎,全球之基因體研究重點轉向人類基因體之定序工作。目前已經發表有完整之第二十一、二十二號兩個染色體之完成序列 (finished sequence),以及其它染色體之初稿序列 (draft sequence),這些基因體資訊提供未來生物醫學研究之重要基礎。本人實驗室自1998年開始規劃台灣之大規模基因體定序能力,建立年產千萬鹼基 (10 Mb)之核心設施,截至目前已對公眾資料庫提供1200萬以上高精確度之序列,主要集中於人類第四號染色體4q21-24 區域,此區域在肝癌病患肝臟細胞基因體經常出現缺失。這些成果可參考網頁資料(網址 http://genome.ym.edu.tw)。

    在4q21-24 區域中,除了可能之抑癌基因 (tumor suppressor gene) 外,尚有上百個基因,其中有些與人類之疾病有關。例如 PKD2基因與體染色體顯性之成年型多囊腎疾病(polycystic kidney disease)有關,SNCA 基因與巴金森氏症(Parkinson disease)有關。這些基因具有重要之醫學意義,值得進一步探討。由於基因表現調控受到染色體局部及整體環境之影響,人類基因體序列除了可以協助發現基因(gene discovery)外,亦對研究基因表現之調控機制提供新的線索。本實驗室完成人類ADH 基因與小鼠Adh基因之比較分析,找出控制整個第一型ADH基因座(Class I ADH gene complex)之關鍵區域。目前正以轉殖鼠技術與生化學方法進一步研究基因調控之機制。

    基因體科技之進展逐漸與臨床醫學結合,對於疾病之診斷、分類、治療與預防作出貢獻。本人實驗室已建立基因體分析技術,例如微陣列及突變 分析之標準程序,將可找出與疾病相關之基因,並進一步剖析基因變異造成人類疾病之病理過程。這些資訊的取得雖然費時耗力,但將可加速對人 類疾病之掌握,甚至帶動對於病人治療方式之新的突破。

    由於基因的運作原理是演化保留下的生物基本法則,本實驗室運用此一觀念找尋人類與果蠅共通之基因並研究其功能。目前已發現數個基因與調 節染色質結構及基因表現有關,目前正進一步探討其生理功能。

  • 教授科目
  • 基因體學
  • 經歷
  • 2006-present
    國立陽明大學
    生命科學系暨基因體科學研究所
    合聘教授
  • 2000-present
    國家衛生研究院
    分子與基因醫學研究所
    特聘研究員
  • 2000-2007
    國家衛生研究院
    分子與基因醫學研究所
    所長
  • 1997-2006
    國立陽明大學遺傳學研究所
    合聘教授
  • 1994-1997
    國立陽明大學遺傳學研究所
    副教授
  • 1991-1994
    國立陽明醫學院遺傳學研究所
    副教授
  • 1988-1990
    波士頓兒童醫院血液腫瘤學研究所
    研究員
  • 1988-1990
    哈佛醫學院小兒科
    研究員
  • 1987-1988
    紐約西奈山醫學中心
    醫學遺傳學研究員
  • 學歷
  • 1983-1987
    美國紐約市立大學西奈山醫學院
    哲學 博士
  • 1974-1981
    臺北醫學院
    醫學士

近五年代表 著作

  • Chang YT, Lin CH, Lee CT, Lin MW, Liu LY, Chen CC, Lee DD, Liu HN, Tsai SF, Matsuura I*. Detection of common mutations in sporadic primary localized cutaneous amyloidosis by DNA mass spectrometry. Br J Dermatol. 2014 Apr;170(4):974-976.
  • Wang JY, Wu PK, Chen PCH, Yen CC, Hung GY, Chen CF, Hung SC, Tsai SF, Liu CL, Chen TH, Chen WM., Manipulation Therapy prior to diagnosis induced primary osteosarcoma metastasis—From clinical to basic research, PLoS ONE. 2014 May 7;9(5):Article number e96571.
  • Yang RC, Chen MH, Chen PY, Chen CY, Tsai SF, Cheng CK, Sun JS. A mutation of the Col2a1 gene (G1170S) alters the transgenic murine phenotype and cartilage matrix homeostasis. J Formos Med Assoc. 2014 Nov;113(11):803-12.
  • Sun WS, Syu WJ, Ho WL, Lin CN, Tsai SF, Wang SH. SitA contributes to the virulence of Klebsiella pneumoniae in a mouse infection model. Microbes Infect. 2014 Feb;16(2):161-70.
  • Huang TW, Lam I, Chang HY, Tsai SF, Palsson BO, Charusanti P. Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578. BMC Res Notes. 2014 Jan 8;7:13.
  • Lee SH, Li CF, Lin HY, Lin CH, Liu HC, Tsai SF*, Niu DM. High-throughput detection of common sequence variations of Fabry disease in Taiwan using DNA mass spectrometry. Mol Genet Metab. 2014 Apr;111(4):507-12.
  • Chung WH, Chang WC, Lee YS, Wu YY, Yang CH, Ho HC, Chen MJ, Lin JY, Hui RC, Ho JC, Wu WM, Chen TJ, Wu T, Wu YR, Hsih MS, Tu PH, Chang CN, Hsu CN, Wu TL, Choon SE, Hsu CK, Chen DY, Liu CS, Lin CY, Kaniwa N, Saito Y, Takahashi Y, Nakamura R, Azukizawa H, Shi Y, Wang TH, Chuang SS, Tsai SF, Chang CJ, Chang YS, Hung SI; Taiwan Severe Cutaneous Adverse Reaction Consortium; Japan Pharmacogenomics Data Science Consortium. Genetic variants associated with phenytoin-related severe cutaneous adverse reactions. JAMA. 2014 Aug 6;312(5):525-34.
  • Lin PC, Lin JK, Lin CH, Lin HH, Yang SH, Jiang JK, Chen WS, Chou CC, Tsai SF*, Chang SC*. Clinical Relevance of Plasma DNA Methylation in Colorectal Cancer Patients Identified by Using a Genome-Wide High-Resolution Array. Ann Surg Oncol. 2014 Dec 4. [Epub ahead of print]
  • Shiao YM, Chung HJ, Chen CC, Chiang KN, Chang YT, Lee DD, Lin MW, Tsai SF, Matsuura I. MCP-1 as an effector of IL-31 signaling in familial primary cutaneous amyloidosis. J Invest Dermatol. 2013 May;133(5):1375-8.
  • Huang TW, Chen TL, Chen YT, Lauderdale TL, Liao TL, Lee YT, Chen CP, Liu YM, Lin AC, Chang YH, Wu KM, Kirby R, Lai JF, Tan MC, Siu LK, Chang CM, Fung CP, Tsai SF*. Copy Number Change of the NDM-1 sequence in a multidrug-resistant Klebsiella pneumoniae clinical isolate. PLoS One. 2013 Apr 29;8(4):e62774.
  • Chen LC, Liu MY, Hsiao YC, Choong WK, Wu HY, Hsu WL, Liao PC, Sung TY, Tsai SF, Yu JS, Chen YJ. Decoding the disease-associated proteins encoded in the human chromosome 4. J Proteome Res. 2013 Jan 4;12(1):33-44.
  • Yang RC, Chen MH, Chen PY, Chen CY, Tsai SF, Cheng CK, Sun JS. A mutation of the Col2a1 gene (G1170S) alters the transgenic murine phenotype and cartilage matrix homeostasis. J Formos Med Assoc. 2013 Oct 26.doi: pii:S0929-6646(13)00355-0.
  • Sun WS, Syu WJ, Ho WL, Lin CN, Tsai SF, Wang SH. SitA contributes to the virulence of Klebsiella pneumoniae in a mouse infection model. Microbes Infect. 2013 Nov 7. pii: S1286-4579(13)00221-9.
  • Ko AM, Tu HP, Liu TT, Chang JG, Yuo CY, Chiang SL, Chang SJ, Liu YF, Ko AM, Lee CH, Lee CP, Chang CM, Tsai SF, Ko YC. ALPK1 genetic regulation and risk in relation to gout. Int J Epidemiol. 2013 Apr;42(2):466-74.
  • Huang YH, Wu HY, Wu KM, Liu TT, Liou RF, Tsai SF, Shiao MS, Ho LT, Tzean SS, Yang UC. Generation and analysis of the expressed sequence tags from the mycelium of Ganoderma lucidum. PLoS One. 2013 May 2;8(5):e61127.
  • Huang TW, Wang JT, Lauderdale TL, Liao TL, Lai JF, Tan MC, Lin AC, Chen YT, Tsai SF, Chang SC. Complete Sequences of Two Plasmids in a blaNDM-1-Positive Klebsiella oxytoca Isolate from Taiwan. Antimicrob Agents Chemother. 2013 Aug;57(8):4072-6.
  • Huang TW, Chen FJ, Miu WC, Liao TL, Lin AC, Huang IW, Wu KM, Tsai SF, Chen YT, Lauderdale TL. Complete genome sequence of Staphylococcus aureus M013, a pvl-positive, ST59-SCCmec type V strain isolated in Taiwan. (2012) J Bacteriol. 194(5):1256-7
  • Lu JW, Hsia Y, Yang WY, Lin YI, Li CC, Tsai TF, Chang KW, Shieh GS, Tsai SF, Wang HD, Yuh CH. Identification of the common regulators for hepatocellular carcinoma induced by hepatitis B virus X antigen in a mouse model. (2012) Carcinogenesis. 33(1):209-19.
  • Lin CH, Lin JK, Chang SC, Chang YH, Chang HM, Liu JH, Li LH, Chen YT, Tsai SF, Chen WS. Molecular profile and copy number analysis of sporadic colorectal cancer in Taiwan. (2011) J Biomed Sci. 18:36.
  • Shu HW, Liu TT, Chan HI, Liu YM, Wu KM, Shu HY, Tsai SF, Hsiao KJ, Hu WS, Ng WV. Genome sequence of the repetitive-sequence-rich Mycoplasma fermentans strain M64. (2011) J Bacteriol. 193(16):4302-3.
  • Huang SW, Lin YY, You EM, Liu TT, Shu HY, Wu KM, Tsai SF, Lo CF, Kou GH, Ma GC, Chen M, Wu D, Aoki T, Hirono I, Yu HT. Fosmid library end sequencing reveals a rarely known genome structure of marine shrimp Penaeus monodon. (2011) BMC Genomics. 12:242.
  • Hsieh KS, Lai TJ, Hwang YT, Lin MW, Weng KP, Chiu YT, Ho TY, Chen CS, Shiue YL, Hsiao M, Tsai SF, Ger LP. IL-10 promoter genetic polymorphisms and risk of Kawasaki disease in Taiwan. (2011) Dis Markers. 30(1):51-9.
  • Naruo Y, Nagashima T, Ushikoshi-Nakayama R, Saeki Y, Nakakuki T, Naka T, Tanaka H, Tsai SF, Okada-Hatakeyama M. Epidermal growth factor receptor mutation in combination with expression of MIG6 alters gefitinib sensitivity. (2011) BMC Syst Biol. 5:29.
  • Liao YC, Huang TW, Chen FC, Charusanti P, Hong JS, Chang HY, Tsai SF, Palsson BO, Hsiung CA. An experimentally validated genome-scale metabolic reconstruction of Klebsiella pneumoniae MGH 78578, iYL1228. (2011) J Bacteriol. 193(7):1710-7.
  • Rui Z, Petrícková K, Skanta F, Pospísil S, Yang Y, Chen CY, Tsai SF, Floss HG, Petrícek M, Yu TW. Biochemical and genetic insights into asukamycin biosynthesis. (2010) J Biol Chem. 285(32):24915-24.
  • Shiao YM, Lee CC, Hsu YH, Huang SF, Lin CY, Li LH, Fann CS, Tsai CY, Tsai SF, Chiu HC. Ectopic and high CXCL13 chemokine expression in myasthenia gravis with thymic lymphoid hyperplasia. (2010) J Neuroimmunol. 221(1-2):101-6.
  • Kuo HP, Lee DF, Chen CT, Liu M, Chou CK, Lee HJ, Du Y, Xie X, Wei Y, Xia W, Weihua Z, Yang JY, Yen CJ, Huang TH, Tan M, Xing G, Zhao Y, Lin CH, Tsai SF, Fidler IJ, Hung MC. ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway. (2010) Sci Signal. 3(108):ra9.
  • Lee HT, Shiao YM, Wu TH, Chen WS, Hsu YH, Tsai SF, Tsai CY. Serum BLC/CXCL13 concentrations and renal expression of CXCL13/CXCR5 in patients with systemic lupus erythematosus and lupus nephritis. (2010) J Rheumatol. 37(1):45-52.
  • Lin MW, Lee DD, Liu TT, Lin YF, Chen SY, Huang CC, Weng HY, Liu YF, Tanaka A, Arita K, Lai-Cheong J, Palisson F, Chang YT, Wong CK, Matsuura I, McGrath JA, Tsai SF. Novel IL31RA gene mutation and ancestral OSMR mutant allele in familial primary cutaneous amyloidosis. (2010) Eur J Hum Genet. 18(1):26-32.
  • Shu HY, Fung CP, Liu YM, Wu KM, Chen YT, Li LH, Liu TT, Kirby R, Tsai SF. Genetic diversity of capsular polysaccharide biosynthesis in Klebsiella pneumoniae clinical isolates. Microbiology. (2009) Microbiology.155(Pt 12):4170-83.
  • Lin MW, Lee DD, Liu TT, Lin YF, Chen SY, Huang CC, Weng HY, Liu YF, Tanaka A, Arita K, Lai-Cheong J, Palisson F, Chang YT, Wong CK, Matsuura I, McGrath JA, Tsai SF. Novel IL31RA gene mutation and ancestral OSMR mutant allele in familial primary cutaneous amyloidosis. Eur J Hum Genet. (2009) Mol Cell. 36(1):131-40.
  • Chen YT, Liao TL, Wu KM, Lauderdale TL, Yan JJ, Huang IW, Lu MC, Lai YC, Liu YM, Shu HY, Wang JT, Su IJ, Tsai SF. Genomic diversity of citrate fermentation in Klebsiella pneumoniae. (2009) BMC Microbiol. 9:168.
  • Nagashima T, Ushikoshi-Nakayama R, Suenaga A, Ide K, Yumoto N, Naruo Y, Takahashi K, Saeki Y, Taiji M, Tanaka H, Tsai SF, Hatakeyama M. Mutation of epidermal growth factor receptor is associated with MIG6 expression. (2009) FEBS J. 276(18):5239-51.
  • Chen YF, Kao CH, Chen YT, Wang CH, Wu CY, Tsai CY, Liu FC, Yang CW, Wei YH, Hsu MT, Tsai SF, Tsai TF. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice. (2009) Genes Dev. 23(10):1183-94.
  • Wu KM, Li LH, Yan JJ, Tsao N, Liao TL, Tsai HC, Fung CP, Chen HJ, Liu YM, Wang JT, Fang CT, Chang SC, Shu HY, Liu TT, Chen YT, Shiau YR, Lauderdale TL, Su IJ, Kirby R, Tsai SF. Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis. (2009) J Bacteriol. 191(14):4492-501.
  • Chen YT, Liao TL, Liu YM, Lauderdale TL, Yan JJ, Tsai SF. Mobilization of qnrB2 and ISCR1 in plasmids. (2009) Antimicrob Agents Chemother. 53(3):1235-1237.
  • Shiao YM, Chang YH, Liu YM, Li JC, Su JS, Liu KJ, Liu YF, Lin MW, Tsai SF. Dysregulation of GIMAP genes in non-small cell lung cancer. (2008) Lung Cancer 62(3):287-294.
  • Hsieh PF, Lin TL, Lee CZ, Tsai SF, Wang JT. Serum-Induced Iron-Acquisition Systems and TonB Contribute to Virulence in Klebsiella pneumoniae Causing Primary Pyogenic Liver Abscess. (2008) J Infect Dis. 197(12):1717-1727.
  • Chang JW, Liu HP, Hsieh MH, Fang YF, Hsieh MS, Hsieh JJ, Chiu YT, Tsai HY, Chen YH, Chen YT, Hsu HY, Chen YT, Tsai SF, Chen YR, Hsi BL, Huang SF. Increased epidermal growth factor receptor (EGFR) gene copy number is strongly associated with EGFR mutations and adenocarcinoma in non-small cell lung cancers: A chromogenic in situ hybridization study of 182 patients. (2008) Lung Cancer 61:328-339.
  • Lin WH, Song JS, Chang TY, Chang CY, Fu YN, Yeh CL, Wu SH, Huang YW, Fang MY, Lien TW, Hsieh HP, Chao YS, Huang SF, Tsai SF, Wang LM, Hsu JT, Chen YR. A cell-based high-throughput screen for epidermal growth factor receptor pathway inhibitors. (2008) Anal Biochem. 377(1):89-94.
  • Dou HY, Tseng FC, Lu JJ, Jou R, Tsai SF, Chang JR, Lin CW, Miu WC, Su IJ. Associations of Mycobacterium tuberculosis genotypes with different ethnic and migratory populations in Taiwan. (2008) Infect Genet Evol. 8(3):323-330.
  • Furuichi T, Maeda K, Chou CT, Liu YF, Liu TC, Miyamoto Y, Takahashi A, Mori K, Ikari K, Kamatani N, Kurosawa H, Inoue H, Tsai SF, Ikegawa S. Association of the MSX2 gene polymorphisms with ankylosing spondylitis in Japanese. (2008) J Hum Genet 53(5):419-424.
  • Lee CT, Amaro C, Wu KM, Valiente E, Chang YF, Tsai SF, Chang CH, Hor LI. A common virulence plasmid in biotype 2 Vibrio vulnificus and its dissemination aided by a conjugal plasmid. (2008) J Bacteriol. 190(5):1638-1648.
  • Lin TL, Lee CZ, Hsieh PF, Tsai SF, Wang JT.Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess. (2008) J Bacteriol. 190(2):515-626.
  • Wu JH, Wu AM, Tsai CG, Chang XY, Tsai SF, Wu TS.Contribution of fucose-containing capsules in Klebsiella pneumoniae to bacterial virulence in mice. (2008) Exp Biol Med (Maywood) 233(1):64-70.
  • Mayhew GF, Bartholomay LC, Kou HY, Rocheleau TA, Fuchs JF, Aliota MT, Tsao IY, Huang CY, Liu TT, Hsiao KJ, Tsai SF, Yang UC, Perna NT, Cho WL, Christensen BM, Chen CC.Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus. (2007) BMC Genomics. 8:462.
  • Fu YN, Yeh CL, Cheng HY, Yang CH, Tsai SF, Huang SF, Chen YR. EGFR mutants found in non-small cell lung cancer show different levels of sensitivity to suppression of Src: implications in targeting therapy. (2008) Oncogene 27(7):957-965.
  • Chen YT, Lauderdale T, Liao TL, Shiau YR, Shu HY, Wu KM, Yan JJ, Su IJ, Tsai SF. Sequencing and Comparative Genomic Analysis of pK29, a 269-kb Conjugative Plasmid Encoding CMY-8 and CTX-M-3 beta-lactamases from Klebsiella pneumoniae. (2007) Antimicrob Agents Chemother. 51(8):3004-3007.
  • Chung WJ, Shu HY, Lu CY, Wu CY, Tseng YH, Tsai SF, Lin CH. Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17. (2007) Proteomics 7(12):2047-2058.
  • Chang YT, Chou CT, Yu CW, Lin MW, Shiao YM, Chen CC, Huang CH, Lee DD, Liu HN, Wang WJ, Tsai SF. Cytokine gene polymorphisms in Chinese patients with psoriasis. (2007) Br J Dermatol. 156(5):899-905.
  • Chang YT, Hsu CY, Chou CT, Lin MW, Shiao YM, Tsai CY, Yu CW, Shiue JJ, Lee YF, Huang CH, Chen CC, Lee DD, Wang WJ, Liu HN, Tsai SF. The genetic polymorphisms of POU5F1 gene are associated with psoriasis vulgaris in Chinese. (2007) J Dermatol Sci. 46(2):153-156.
  • Wang HY, Chien HC, Osada N, Hashimoto K, Sugano S, Gojobori T, Chou CK, Tsai SF, Wu CI, Shen CK. Rate of Evolution in Brain-Expressed Genes in Humans and Other Primates. (2007) PLoS Biol. 5(2):e13.
  • Hung KW, Lin YC, Cheng CC, Fan PJ, Chang CF, Tsai SF, Huang TH. Resonance assignments of a CoA binding protein from Klebsiella pneumoniae. (2006) J Biomol NMR. 36(5):74.
  • Chen YT, Shu HY, Li LH, Liao TL, Wu KM, Shiau YR, Yan JJ, Su IJ, Tsai SF, Lauderdale TL. Complete Nucleotide Sequence of pK245, a 98-Kilobase Plasmid Conferring Quinolone Resistance and Extended-Spectrum-{beta}-Lactamase Activity in a Clinical Klebsiella pneumoniae Isolate. (2006) Antimicrob Agents Chemother. 50(11):3861-3866.
  • Lee DD, Lin MW, Chen IC, Huang CY, Liu MT, Wang CR, Chang YT, Liu HN, Liu TT, Wong CK, Tsai SF. Genome-Wide Scan Identifies a Susceptibility Locus for Familial Primary Cutaneous Amyloidosis on Chromosome 5p13.1-q11.2. (2006) Br J Dermatol. 155(6):1201-8.
  • Chang YT, Chou CT, Shiao YM, Lin MW, Yu CW, Chen CC, Huang CH, Lee DD, Liu HN, Wang WJ, Tsai SF. Psoriasis vulgaris in Chinese is associated with PSORS1C3 and CDSN genes. (2006) Br J Dermatol. 155(4):663-669.
  • Chang YT, Chou CT, Shiao YM, Lin MW, Yu CW, Chen CC, Huang CH, Lee DD, Liu HN, Wang WJ, Tsai SF. The killer cell immunoglobulin-like receptor genes do not confer susceptibility to psoriasis vulgaris independently in Chinese. (2006) J Invest Dermatol. 126(10):2335-2338.
  • Hsieh MH, Fang YF, Chang WC, Kuo HP, Lin SY, Liu HP, Liu CL, Chen HC, Ku YC, Chen YT, Chang YH, Chen YT, Hsi BL, Tsai SF, Huang SF. Complex Mutation Patterns of Epidermal Growth Factor Receptor Gene Associated with Variable Responses to Gefitinib Treatment in Patients with Non-Small Cell Lung Cancer. (2006) Lung Cancer 53(3):311-322.
  • Su JS, Tsai TF, Chang HM, Chao KM, Su TS, Tsai SF. Distant HNF1 site as a master control for the human class I alcohol dehydrogenase gene expression. (2006) J Biol Chem. 281:19809-19821.
  • Chang YT, Lee WR, Yu CW, Liu HN, Lin MW, Huang CH, Chen CC, Lee DD, Wang WJ, Hu CH, Tsai SF. No association of cytokine gene polymorphisms in Chinese patients with atopic dermatitis. (2006) Clin Exp Dermatol. 31:419-423.
  • Chen YR, Fu YN, Lin CH, Yang ST, Hu SF, Chen YT, Tsai SF, Huang SF. Distinctive activation patterns in constitutively active and gefitinib-sensitive EGFR mutants. (2006) Oncogene. 25(8):1205-1215.
  • Chang YT, Liu HN, Yu CW, Lin MW, Huang CH, Chen CC, Liu MT, Lee DD, Wang WJ, Tsai SF. Cytokine gene polymorphisms in bullous pemphigoid in a Chinese population. (2006) Br J Dermatol. 154(1):79-84.
  • Chou TY, Chiu CH, Li LH, Hsiao CY, Tzen CY, Chang KT, Chen YM, Perng RP, Tsai SF, Tsai CM. Mutation in the tyrosine kinase domain of epidermal growth factor receptor is a predictive and prognostic factor for gefitinib treatment in patients with non-small cell lung cancer. (2005) Clin Cancer Res. 11(10):3750-3757.
  • Liu YF, Chen WM, Lin YF, Yang RC, Lin MW, Li LH, Chang YH, Jou YS, Lin PY, Su JS, Huang SF, Hsiao KJ, Fann CS, Hwang HW, Chen YT, Tsai SF. Type II collagen gene variants and inherited osteonecrosis of the femoral head. (2005) N Engl J Med. 352(22):2294-2301.
  • Lin MW, Lee DD, Lin CH, Huang CY, Wong CK, Chang YT, Liu HN, Hsiao KJ, Tsai SF. Suggestive Linkage of Familial Primary Cutaneous Amyloidosis to a Locus on Chromosome 1q23. (2005) Br J Dermatol. 152(1):29-36.
  • Chang YH, Su WH, Lee TC, Sun HF, Chen CH, Pan WH, Tsai SF, Jou YS. TPMD: a database and resources of microsatellite marker genotyped in Taiwanese populations. (2005) Nucleic Acids Res. Database Issue:D174-177.
  • Tsai HY, Hsi BL, Hung IJ, Yang CP, Lin JN, Chen JC, Tsai SF, Huang SF. Correlation of MYCN amplification with MCM7 protein expression in neuroblastomas: a chromogenic in situ hybridization study in paraffin sections. (2004) Hum Pathol. 35(11):1397-1403.
  • Huang SF, Liu HP, Li LH, Ku YC, Fu YN, Tsai HY, Chen YT, Lin YF, Chang WC, Kuo HP, Wu YC, Chen YR, Tsai SF. High frequency of epidermal growth factor receptor mutations with complex patterns in non-small cell lung cancers related to gefitinib responsiveness in Taiwan. (2004) Clin Cancer Res. 10(24):8195-8203.
  • Yeh SH, Lin MW, Lu SF, Wu DC, Tsai SF, Tsai CY, Lai MY, Hsu HC, Chen DS, Chen PJ. Allelic loss of chromosome 4q21 approximately 23 associates with hepatitis B virus-related hepatocarcinogenesis and elevated alpha-fetoprotein. (2004) Hepatology. 40(4):847-854.
  • Kao WC, Chen YR, Yi EC, Lee H, Tian Q, Wu KM, Tsai SF, Yu SS, Chen YJ, Aebersold R, Chan SI. Quantitative proteomic analysis of metabolic regulation by copper ions in methylococcus capsulatus (Bath). (2004) J Biol Chem. 279(49):51554-51560.
  • Chen YT, Chang HY, Lai YC, Pan CC, Tsai SF, Peng HL. Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43. (2004) Gene 337:189-198.
  • Chang YT, Liu HN, Wang WJ, Lee DD, Tsai SF. A study of cytokeratin profiles in localized cutaneous amyloids. (2004) Arch. Dermatol. Res. 296(2): 83-88.
  • Chen WM, Liu YF, Lin MW, Chen IC, Lin PY, Lin GL, Jou YS, Lin YT, Fann CS, Wu JY, Hsiao KJ & Tsai SF. Autosomal dominant avascular necrosis of femoral head in two Taiwanese pedigrees and linkage to chromosome 12q13. (2004) Am. J. Hum. Genet. 75(2): 310-317.
  • Bartholomay LC, Cho WL, Rocheleau TA, Boyle JP, Beck ET, Fuchs JF, Liss P, Rusch M, Butler KM, Wu RC, Lin SP, Kuo HY, Tsao IY, Huang CY, Liu TT, Hsiao KJ, Tsai SF, Yang UC, Nappi AJ, Perna NT, Chen CC, Christensen BM. Description of the Transcriptomes of Immune Response-Activated Hemocytes from the Mosquito Vectors Aedes aegypti and Armigeres subalbatus. (2004) Infect. Immun. 72(7):4114-4126.
  • Chang YT, Shiao YM, Chin PJ, Liu YL, Chou FC, Wu S, Lin YF, Li LH, Lin MW, Liu HN, Tsai SF. Genetic polymorphisms of the HCR gene and a genomic segment in close proximity to HLA-C are associated with patients with psoriasis in Taiwan. (2004) Br. J. Dermatol. 150(6):1104-1111.
  • The International Chimpanzee Chromosome 22 Consortium: Watanabe H, Fujiyama A, Hattori M, Taylor TD, Toyoda A, Kuroki Y, Noguchi H, BenKahla A, Lehrach H, Kube M, Sudbrak R, Taenzer S, Galgoczy P, Platzer M, Scharfe M, Nordsiek G, Blocker H, Hellmann I, Khaitovich P, Paabo S, Reinhardt R, Zheng HJ, Zhang XL, Zhu GF, Wang BF, Fu G, Ren SX, Zhao GP, Chen Z, Lee YS, Cheong JE, Choi SH, Wu KM, Liu TT, Hsiao KJ, Tsai SF, Kim CG, Oota S, Kitano T, Kohara Y, Saitou N, Park HS, Wang SY, Yaspo ML, Sakaki Y. DNA sequence and comparative analysis of chimpanzee chromosome 22. (2004) Nature 429: 382-388.
  • Yeh SH, Wang HY, Tsai CY, Kao CL, Yang JY, Liu HW, Su IJ, Tsai SF, Chen DS, Chen PJ, The National Taiwan University SARS Research Team, Chen DS, Lee YT, Teng CM, Yang PC, Ho HN, Chen PJ, Chang MF, Wang JT, Chang SC, Kao CL, Wang WK, Hsiao CH, Hsueh PR. Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: Molecular epidemiology and genome evolution. (2004) Proc. Natl. Acad. Sci. USA. 101(8):2542-2547.
  • 3Li LH, Li JC, Lin YF, Lin CY, Chen CY & Tsai SF. Genomic shotgun array: a procedure linking large-scale DNA sequencing with regional transcript mapping. (2004) Nucleic Acids Res. 32(3):E27.
  • Huang CJ, Chen CY, Chen HH, Tsai SF & Choo KB. TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF(TD) and POZ/BTB domains. (2004) Gene 324:117-127.
  • Jong YJ, Li LH, Tsou MH, Chen YJ, Cheng SH, Wang-Wuu S, Tsai SF, Chen CM, Huang AT, Hsu MT & Lin CH. Chromosomal comparative genomic hybridization abnormalities in early- and late-onset human breast cancers: correlation with disease progression and TP53 mutations. (2004) Cancer Genet Cytogenet. 148(1):55-65.
  • Sakaki Y, Watanabe H, Taylor T, Hattori M, Fujiyama A, Toyoda A, Kuroki Y, Itoh T, Saitou N, Oota S, Kim CG, Kitano T, Lehrach H, Yaspo ML, Sudbrak R, Kahla A, Reinhardt R, Kube M, Platzer M, Taenzer S, Galgoczy P, Kel A, Bloecker H, Scharfe M, Nordsiek G, Hellmann I, Khaitovich P, Paabo S, Chen Z, Wang SY, Ren SX, Zhang XL, Zheng HJ, Zhu GF, Wang BF, Zhao GP, Tsai SF, Wu K, Liu TT, Hsiao KJ, Park HS, Lee YS, Cheong JE, Choi SH; Chimpanzee Chromosome 22 Sequencing Consortium. Human versus chimpanzee chromosome-wide sequence comparison and its evolutionary implication. (2003) Cold Spring Harb Symp Quant Biol. 68:455-460.
  • Liu MT*, Su JS*, Huang CY & Tsai SF. Novel Mutations Involving the NF1 Gene Coding Sequence in Neurofibromatosis Type 1 Patients from Taiwan. (2003) J Hum Genet. 48(10):545-549.
  • Chen CY*, Wu KM*, Chang YC, Chang C-H, Tsai HC, Liao TL, Liu YM, Chen HJ, Shen Arthur BT, Li JC, Su TL, Shao CP, Lee CT, Hor LI, & Tsai SF.Comparative genome analysis of Vibrio vulnificus, a marine pathogen. (2003) Genome Research 13: 2577-2587.
  • Chang YT, Tsai SF, Lin MW, Liu HN, Lee DD, Shiao YM, Chin PJ, & Wang WJ. SPR1 gene near HLA-C is unlikely to be a psoriasis susceptibility gene. (2003) Experimental Dermatology. 12:307-314.
  • Chang YT, Tsai SF, Lee DD, Shiao YM, Huang CY, Liu HN, Wang WJ & Wong CK. A study of candidate genes for psoriasis near HLA-C in Chinese patients with psoriasis. (2003) British Journal of Dermatology. 148(3):418-423.
  • Fujiyama A, Watanabe H, Toyoda A, Taylor TD, Itoh T, Tsai SF, Park HS, Yaspo ML, Lehrach H, Chen Z, Fu G, Saitou N, Osoegawa K, de Jong PJ, Suto Y, Hattori M, & Sakaki Y. (2002) Construction and analysis of the first human-chimpanzee comparative clone map. Science 295:131-134.
  • Chang YT, Tsai SF, Wang WJ, Hong CJ, Huang CY, & Wong CK. A study of apolipoproteins E and A-l in cutaneous amyloids. (2001) Br. J. Dermatol. 145:422-427.
  • Shen HH, Huang AM, Hoheisel J, & Tsai SF. Identification and characterization of a SET/NAP protein encoded by the brain-specific gene, MB20. (2001) Genomics 71(1): 21-33.
  • Bressler J, Tsai SF, Wu MY, Tsai, SF, Ramirez MA, Armstrong D, & Beaudet AL. The SNRPN prrmoter is not required for genomic imprinting of the Prader-Willi/Angelman domain in mice. (2001) Nat. Genet. 28:232-240.
  • Tsai SF, & Chen PJ. The impact of human genome research on medicine -- The initial Taiwan Experience. (2000) Journal of the Formosan Medical Association 99(2):107-115.
  • Chen YJ, Yeh SH., Chen JT, Wu CC, Hsu MT, Tsai SF, Chen PJ, & Lin CH. Chromosomal changes and clonality relationship between primary and recurrent hepatocellular carcinoma. (2000) Gastroenterology 119(2):431-440.
  • Chen YJ, Chen PJ, Shu CH, Hsu MT, Tsai SF, & Lin CH. Chromosomal aberrations in nasopharyngeal carcinoma analyzed by comparative genomic hybridization. (1999) Genes Chromosomes and Cancer 25:169-175.
  • Tsou AP, Wu KM, Tseng TI, Chi CW, Chiu JW, Lui WY, Hu CP, Chang C, Chou,CK, & Tsai SF. Parallel hybridization analysis of multiple protein kinase genes: identification of gene expression patterns characteristic of human hepatocellular carcinoma. (1998) Genomics 50: 331-340.
  • Fan FF, Shen HH, Tseng WP, Chen PM, & Tsai SF. Molecular cloning and characterization of a human brain-specific gene implicated in neuronal differentiation. (1998) Molecular Brain Research 54: 113-123.
  • Tsai SF, Jang A CC, Prikhod'Ko G., Bessarab DA, Tang CY, Pflugfelder GO, & Sun YH. (1997) Gypsy retrotransposon as a tool for the in vivo analysis of the regulatory region of the optomotor-blind gene in Drosophila. Proc. Natl. Acad. Sci. USA. 94: 3837-3841.
  • Lee DD, Huang JY, Wong CK., Gagel RF, & Tsai SF. (1996) Genetic heterogeneity of familial primary cutaneous amyloidosis: Lack of evidence for linkage with the chromosome 10 pericentromeric region in Chinese families. J. Inv. Derma. 107: 30-33.
  • Lin WH, Huang LH., Yeh JY, Hoheisel J, Lehrach H, Sun YH, & Tsai SF. (1995) Expression of a Drosophila GATA transcription factor in multiple tissues in the developing embryos. Identification of homozygous lethal mutants with P-element insertion at the promoter region. J. Biol. Chem. 27: 25150-25158.
  • Skoda RC, Tsai SF, Orkin SH, & Leder P. (1995) Expression of c-MYC under the control of GATA-1 Regulatory sequences causes erythroleukemia in transgenic mice. J. Exp. Med. 181: 1603-1613.
  • Pai JT, Tsai SF, Horng CJ, Chiu PC, Cheng MY, Hsiao KJ, & Wu KD. (1994) Absence of FMR-1 gene expression can be detected with RNA extracted from dried blood specimens. Hum. Genet. 93: 488-493.
  • Warner CA, Poh-Fitzpatrick MB, Zaider EF, Tsai SF, & Desnick RJ. (1992) Congenital erythropoietic porphyria, A mild variant with low uroporphyrin I level due to a missense mutation (A66V) encoding residual uroporphyrinogen III synthase activity. Arch. Dermatol. 128: 1243-1248.
  • Astrin KH, Warner CA, Yoo HK, Goodfellow PJ, Tsai SF, & Desnick RJ. (1991) Regional assignment of the human uroporphyrinogen III synthase (UROS) gene to chromosome 10q25.2 -q26.3. Hum. Genet. 87: 18-22.
  • Ho IC, Vorhees P, Marin N, Karpinski Oakley B, Tsai SF, Orkin SH, & Leiden J M. (1991) Human GATA-3: a lineage-restricted transcription factor that regulates the expression of the T cell receptor a gene. EMBO J. 10: 1187-1192.
  • Tsai SF, Strauss E, & Orkin SH. (1991) Functional analysis and in vivo footprinting implicate the erythroid transcription factor GATA-1 as a positive regulator of its own promoter. Genes & Dev. 5: 919-931.
  • Pevny L, Simon MC, Robertson E, Klein WH, Tsai SF, D'Agati V, Orkin SH, & Costantini F. (1991) A targeted mutation in the gene for transcription factor GATA-1 blocks erythroid differentiation in chimeric mice. Nature (London) 349: 257-260.
  • Whitelaw E, Tsai SF, Hogben P, & Orkin SH. (1990) Regulated expression of globin chains and the erythroid transcription factor (GF-1/NF-E1/Eryf1) during erythropoiesis in the developing mouse. Mol. Cell. Biol. 10: 6596-6606.
  • Zon LI, Tsai SF, Burgess S, Matsudaira P, Bruns G.A, & Orkin SH.(1990) The major human erythroid DNA-binding protein (GF-1): Primary sequence and localization of the gene to the X chromosome. Proc. Natl. Acad. Sci. USA. 87, 668-672.
  • Tsai SF, Martin DIK, Zon LI., D'Andrea AD, Wong G.G. & Orkin SH. (1989) Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells. Nature (London) 339, 446-451.
  • Martin DIK, Tsai SF, & Orkin SH. (1989) Increased g-globin expression in a nondeletion HPFH mediated by an erythroid-specific DNA binding factor. Nature (London) 338, 435-438.
  • Tsai SF, Bishop DF, & Desnick RJ. (1988) Human uroporphyrinogen III synthase: molecular cloning, nucleotide sequence, and expression of a full-length cDNA. Proc. Natl. Acad. Sci. USA. 85, 7049-7053.
  • Tsai SF, Bishop DF, & Desnick RJ. (1987) Coupled-enzyme and direct assays for uroporphyrinogen III synthase activity in human erythrocytes and cultured lymphoblasts. Enzymatic diagnosis of heterozygotes and homozygotes with congenital erythropoietic porphyria. Anal. Biochem. 166, 120-133.
  • Tsai SF, Bishop DF, & Desnick RJ. (1987) Purification and properties of uroporphyrinogen III synthase from human erythrocytes. J. Biol. Chem. 262, 1268-1273.