教師簡介

  • 專任助理教授
  • 藍昇輝
  • Sheng-Hui Lan
  • 02-2826-7030 /lab: ext. 6210
  • shlan@ym.edu.tw
  • 圖書資訊暨研究大樓7樓702室
  • 專業領域
  • 細胞自噬功能與調控
    The Role of Autophagy in physiology and pathology
  • 研究目標
  • My long term goal is to reveal novel functions of autophagy in normal cellular physiology and pathology through the analysis of the double-membrane autophagosome and autophagic machinery using cell and animal models.

  • 背景介紹
  • Autophagy regulates cellular metabolism to protect cell from stress-caused damages through degradation machinery. Over 20 autophagy related genes (Atg) are responsible for two major ubiquitination-like conjugation systems. Accumulating evidence shows that autophagosome selectively recruits cargos (aggregated protein, damaged organelle, pathogen and microRNA) in cytosol, followed by fusion with the lysosome for degradation. In our studies, we are the first to reveal that autophagosome selectively recruit oncogenic microRNA (miR-224) and protein (Cyclin D1) to regulate the tumorigenesis of hepatocellular carcinoma. These data indicate that autophagosome recognizes and recruits specific molecules followed by degradation.

    Another function of autophagy called secretory autophagy (also called autosecretion or type III secretion) is getting more attention. Different from the traditional degradative autophagy, the recruited cytosolic molecules are carried by the double-membrane autophagosome and exported to extracellular environment. However, physiological and pathological role of secretory autophagy remains poorly understood.

  • 研究方向
  • 1. Identification of the cargos delivered to extracellular environment through secretory autophagy.
    2. Clarification of the relationship between degradative and secretory autophagy.
    3. Regulation of secretory autophagy for the therapy of tumorigenesis, diabetes mellitus and other diseases.
    We have identified a group of the proteins related to the vesicle-mediated transportation as well as the extracellular cargos by LC/MS/MS proteomics analysis. In the future, we would like to validate these candidate proteins and clarify their functions under normal physiological and pathogenic conditions. For cancer studies, we have established several orthotropic cancer models (liver, lung and bladder) and metastatic animal models (lung to lung and tail vein). For diabetes mellitus, streptozotocin (STZ) induced type-I DM and high fat diet induced type-II DM mice models were also developed in this lab.

  • 教授科目
  • 生命科學總論(下)
  • 經歷
  • Feb, 2019-present
    國立陽明大學
    生命科學系暨基因體科學研究所
    助理教授
  • Aug, 2018-Jan, 2019
    中國醫藥大學
    生物科技學系暨碩博士班
    助理教授
  • Aug 2017- Jul 2018
    國立成功大學
    生物化學科暨分子生物學研究所
    博士後研究員
  • Feb 2017-Jul 2017
    美國耶魯大學醫學院
    細胞生物學研究所
    訪問學者
  • Dec 2013-Jan 2017
    國立成功大學
    微生物及免疫學研究所
    博士後研究員
  • 學歷
  • 2013
    國立成功大學基礎醫學研究所
    博士
  • 2007
    中國醫藥大學營養學研究所
    碩士
  • 2005
    中國醫藥大學營養學系
    學士

代表著作

  • Wu SY*, Lan SH*, Liu HS. Degradative autophagy selectively regulates CCND1 (cyclin D1) and MIR224, two oncogenic factors involved in hepatocellular carcinoma tumorigenesis. Autophagy 2019: (SCI). (Impact factor: 11.1;Rank: 6.58%)第一作者
  • Wu SY*, Lan SH*, Wu SR, Chiu YC, Lin XZ, Su IJ, Tsai TF, Yen CJ, Lu TH, Liang FW, Li CY, Su HJ, Su CL, Liu HS. Hepatocellular carcinoma-related cyclin D1 is selectively regulated by autophagy degradation system. Hepatology 2018; 68(1):141-154 (SCI). (Impact factor: 14.079;Rank: 5.63%)第一作者
  • Kao YT, Wu CH, Wu SY, Lan SH, Liu HS, Tseng YS. Arsenic treatment increase Aurora-A overexpression through E2F1 activation in bladder cells. BMC CANCER 2017; 17(1): (SCI). (Impact factor: 3.288;Rank: 47.76%)
  • Wu SY*, Lan SH*, Liu HS. Autophagy and microRNA in hepatitis B virus-related hepatocellular carcinoma. WORLD JOURNAL OF GASTROENTEROLOGY 2016; 22(1):176-87 (SCI). (Impact factor: 3.3;Rank: 43.13%)第一作者
  • Yeh HH, Tseng YF, Hsu YC,Lan SH, Wu SY, Raghavaraju G, Cheng DE, Lee YR, Chang TY, Chow NH, Hung WC, Liu HS. Ras induces experimental lung metastasis through up-regulation of RbAp46 to suppress RECK promoter activity. BMC CANCER 2015; 25(15): (SCI). (Impact factor: 3.288;Rank: 47.76%)
  • Won SJ, Yen CH, Liu HS, Wu SY, Lan SH, Jiang-Shieh YF, Lin CN, Su CL. Justicidin A-Induced Autophagy Flux Enhances Apoptosis of Human Colorectal Cancer Cells via Class III PI3K and Atg5 Pathway. JOURNAL OF CELLULAR PHYSIOLOGY 2015; 230(4):930-46 (SCI). (Impact factor: 3.923;Rank: 15.06%)
  • Lan SH, Wu SY, Zuchini R, Lin XZ, Su IJ, Tsai TF, Lin YJ, Wu CT, Liu HS. Autophagy suppresses tumorigenesis of hepatitis B virus-associated hepatocellular carcinoma through degradation of miR-224. Hepatology 2014; 59(2):505-17 (SCI). (Impact factor: 14.079;Rank: 5.63%)第一作者
  • Lan SH, Wu SY, Zuchini R, Lin XZ, Su IJ, Tsai TF, Lin YJ, Wu CT, Liu HS. Autophagy-preferential degradation of miR-224 participates in hepatocellular carcinoma tumorigenesis. Autophagy 2014; 10(9):1687-9 (SCI). (Impact factor: 11.1;Rank: 6.58%)第一作者
  • Ip SW, Lan SH, Huang AC, Yang JS, Chen YY, Huang HY, Lin ZP, Hsu YM, Yang MD, Chiu CF, Chung JG. Capsaicin induces apoptosis in SCC-4 human tongue cancer cells through mitochondria-dependent and -independent pathways. ENVIRONMENTAL TOXICOLOGY 2012; 27(6):332-41 (SCI). (Impact factor: 2.491;Rank: 23.89%)第二作者
  • Ip SW, Lan SH, Lu HF, Huang AC, Yang JS, Lin JP, Huang HY, Lien JC, Ho CC, Chiu CF, Wood W, Chung JG. Capsaicin mediates apoptosis in human nasopharyngeal carcinoma NPC-TW 039 cells through mitochondrial depolarization and endoplasmic reticulum stress. HUMAN & EXPERIMENTAL TOXICOLOGY 2012; 31(6):539-49 (SCI). (Impact factor: 1.84;Rank: 73.94%)第二作者
  • Wu SY, Lan SH, Cheng DE, Chen WK, Shen CH, Lee YR, Zuchini R, Liu HS. Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation. NEOPLASIA 2011; 13(12):1171-82 (SCI). (Impact factor: 4.994;Rank: 22.20%)第二作者
  • Hsieh CC, Wu SY, Lan SH, Weng TY, Tsai Y, Liu HS. Effect of Ha-rasval12 on nm23 expression, tumor formation and metastasis of the transformants, and immunomodulation in tumor-bearing mice. ANTICANCER RESEARCH 2010; 30(9):3585-92 (SCI). (Impact factor: 1.865;Rank: 81.84%)