教師簡介

  • 兼任教授
  • 蔡宜芳
  • Yi-Fang Tsay
  • 02-2789-9198
  • yftsay@gate.sinica.edu.tw
  • 中研院分生所N317
  • 實驗室網頁
  • http://www.imb.sinica.edu.tw/~mbyftsay/
  • 專業領域
  • Nitrate Transport and Sensing in Higher Plants
  • 研究方向
  • I. NRT1(PTR) 基因群的功能研究

    硝酸鹽是大多數植物最主要的氮源。有效率的硝酸鹽吸收與輸送對植物的生長很重要。硝酸鹽被植物吸收後,可以立刻被根部組織所代謝、也可 以儲存在液泡中以供未來之所需,或輸送到葉部組織在加以代謝。

    針對 NRT1( PTR) family中的硝酸鹽轉運蛋白作深入的探討,我們瞭解到阿拉伯芥中硝酸鹽的吸收與輸送到不同組織的分子機制與調控方式。

    II. 硝酸鹽的訊息傳導機制

    植物可以探知硝酸鹽的濃度高低而調控發育生長及基因的表現。CHL1是一個雙親和性的轉運蛋白。我們發現CHL1是藉由磷酸化,在高、 低親和性之間做轉換。當CHL1被磷酸化的時候,它是一個高親和性的硝酸鹽轉運蛋白;去磷酸化之後,它轉換成低親和性的轉運蛋白。我們新 的研究顯示CHL1也是硝酸鹽的感受器,藉由蛋白激脢CIPK23及去磷酸脢PP2C調控磷酸化。藉由這些蛋白質之間的多樣性交互作用, 植物可以因應硝酸鹽濃度的變化或時間上的變化而誘發不同程度的反應。

    III. NUE : 作物的氮利用效率

    氮肥增加作物的產量,但是氮肥的生產消耗百分之一的世界能源。有一半以上的氮肥無法被植物吸收,反而污染水源且產生溫室效應氣體。 NRT1 硝酸鹽轉運蛋白提供了改良作物氮利用效率的新工具。另外我們也利用不同的生態品系尋找影響植物氮利用效率的相關基因。


  • 經歷
  • -present
    國立陽明大學
    生命科學系暨基因體科學研究所
    兼任教授
  • Feb, 2016-present
    中央研究院分子生物研究所
    特聘研究員
  • Mar, 2009-Feb, 2016
    中央研究院分子生物研究所
    研究員
  • May 1999-Mar, 2009
    中央研究院分子生物研究所
    副研究員
  • Feb, 1999-May 1999
    中央研究院分子生物研究所
    助研究員
  • 1990-1993
    美國加州大學聖地牙哥分校生物系
    博士後研究員
  • 學歷
  • 1990
    美國卡內基美隆大學
    生物學 博士
  • 1985
    國立臺灣大學
    植物學研究所 碩士
  • 1983
    國立臺灣大學
    植物學系 學士

代表著作

  • N. C.Huang, C. S. Chiang, N. M. Crawford,. and Y. F. Tsay* (1996) CHL1 Encodes a Component of the Low-Affinity Nitrate Uptake System in Arabidopsis and Shows Cell Type-Specific Expression in Roots. Plant Cell 8(12):2183-2191. [pdf]
  • M. J. Frank, D. Liu, Y. F. Tsay, C. Ustach and N. M. Crawford (1997) Tag1 is an Autonomous Transposable Element That Shows Somatic Excision in Both Arabidopsis and Tobacco. Plant Cell 9(10):1745-1756. [pdf]
  • K.-H. Liu, C.-Y. Huang and Y.-F. Tsay* (1999) CHL1 is a Dual-Affinity Nitrate Transporter of Arabidopsis Involving in Multiple Phases of Nitrate Uptake. Plant Cell 11(5):865-874. [pdf]
  • N.-C. Huang, K.-H. Liu, H.-J. Ho and Y.-F. Tsay* (1999) Cloning and Functional Characterization of an Arabidopsis Nitrate Transporter Gene That Encodes a Constitutive Component of Low-Affinity Uptake. Plant Cell 11(8):1381-1392. [pdf]
  • C.-M. Lin, S. Koh, G. Stacey, S.-M. Yu, T.-Y. Lin and Y.-F. Tsay*. (2000) Cloning and Functional Characterization of a Constitutively Expressed Nitrate Transporter Gene, OsNRT1, from rice. Plant Physiol.122:379-388, 2000. [pdf]
  • K.-H. Liu and Y.-F. Tsay*. (2003) Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation. EMBO J.22:1005-1013. [pdf]
  • J. Jeong, S. Suh, C. Guan, Y.-F. Tsay, N. Moran, J. Chang, C.-S. An, K.N. Demchenko, K. Pawlowski and Y. Lee. (2004) A Nodule-Specific Dicarboxylate Transporter from Alder Is a Member of the Peptide Transporter Family. Plant Physiol.134:969-978. [pdf]
  • C. S. Chiang, G. Stacey and Y.-F. Tsay*. (2004) Mechanisms and Functional Properties of Two Peptide Transporters, AtPTR2 and fPTR2. J Biol Chem.279:30150-30157. [pdf]
  • C. C. Chiu, C. S. Lin, A. P. Hsia, R. C. Su, H. L. Lin and Y.-F. Tsay*. (2004) Mutation of a Nitrate Transporter, AtNRT1:4, Results in a Reduced Petiole Nitrate Content and Altered Leaf Development. Plant Cell Physiol.45:1139-1148.
  • Y.-F. Tsay*, C.-C. Chiu , C.-B. Tsai, C.-H. Ho and P.-K. Hsu (2007) Nitrate transporters and peptide transporters. FEBS Lett. 581(12):2290-300. Review. [pdf]
  • S.-H. Lin, H.-F. Kuo, G. Canivenc, C.-S. Lin, M. Lepetit, P.-K. Hsu, P. Tillard, H.-L. Lin, Y.-Y. Wang, C.-B. Tsai and Y.-F. Tsay* (2008) Mutation of the Arabidopsis NRT1.5 Nitrate Transporter Causes Defective Root-to-Shoot Nitrate Transport. Plant Cell 20(9): 2514-2528. (Recommended by Faculty1000) [pdf]
  • A. Almagro, S.-H. Lin and Y.-F. Tsay* (2008) Characterization of the Arabidopsis Nitrate Transporter NRT1.6 Reveals a Role of Nitrate in Early Embryo Development. Plant Cell 20(12):3289-3299. [pdf]
  • H.-C. Hu, Y.-Y. Wang and Y.-F. Tsay* (2009) AtCIPK8, a CBL-interacting protein kinase, regulates the low-affinity phase of the primary nitrate response. Plant J. 57(2):264-78. [pdf]
  • C.-H. Ho, S.-H. Lin, H.-C. Hu, and Y.-F. Tsay* (2009) CHL1 Functions as a Nitrate Sensor in Plants. Cell 138, 1184–1194. (Recommended by Faculty1000, 12 stars) [pdf]
  • S.-C. Fan, C.-S. Lin, P.-K. Hsu, S.-H. Lin, and Y.-F. Tsay* (2009) The Arabidopsis Nitrate Transporter NRT1.7, Expressed in Phloem, Is Responsible for Source-to-Sink Remobilization of Nitrate. Plant Cell 21: 2750–2761. [pdf]
  • G. Krouk*, N. M. Crawford, G. M. Coruzzi, Y.-F. Tsay* (2010) Nitrate signalling: adaptation to fluctuating environments. Curr. Opin. Plant Biol. 13(3):266-73. Review. [pdf]
  • C.-H. Ho and Y.-F. Tsay* (2010) Nitrate, ammonium, and potassium sensing and signaling. Curr. Opin. Plant Biol. 13(5):604-10. Review. [pdf]
  • Y.-F. Tsay* and P.-K. Hsu (2011) The Role of Plasma Membrane Nitrogen Transporters in Nitrogen Acquisition and Utilization. Plant Cell Monographys. 19: 223-236. Review [pdf]
  • Y.-F. Tsay*, C.-H. Ho, H.-Y. Chen, and S.-H. Lin (2011) Integration of Nitrogen and Potassium Signaling. Annu. Rev. Plant Biol. 62: 207-226. [pdf]
  • Y.-Y. Wang and Y.-F. Tsay* (2011) Arabidopsis Nitrate Transporter NRT1.9 is Important in Phloem Nitrate Transport. Plant Cell 23: 1945-1957. [pdf]
  • Y.-Y. Wang, P.-K. Hsu and Y.-F. Tsay* (2012) Uptake, allocation and signaling of nitrate. Trends in Plant Science 17:1360-1385. [pdf]
  • J. I. Schroeder *, E. Delhaize, W. B. Frommer, M. Lou Guerinot, M. J. Harrison, L. Herrera-Estrella, T. Horie, L. V. Kochian, R. Munns, N. K. Nishizawa, Y.-F. Tsay, D. Sanders* (2013) Using membrane transporters to improve crops for sustainable food production. Nature 497 (7447):60-66. [pdf]